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Although repurposing existing drugs offers a cost-effective and time-efficient alternative for identifying new therapies, their effective concentrationsmeasured as the half-maximal inhibitory concentration (IC50)often exceed the maximum safe plasma levels in humans, creating a major barrier to clinical application

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To protect against the harmful effects of OS and maintain metabolic homeostasis, the brain (like the rest of the body) uses antioxidants such as glutathione, superoxide dismutase, catalase, and vitamins C and E to neutralize excess ROS (Dwivedi et al., 2020)

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Subtle changes in ROS levels can mediate signaling functions such as responses to environmental changes [37,52,56,57] which can involve modulation of the redox state of protein thiols [58] as discussed below